T4 DNA Ligase

- Overview
  - T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates.
    This is a high-quality (commercial grade) version of the T4 DNA Ligase. T4 DNA Ligase is recombinantly produced in E. coli. ArcticZymes’ T4 DNA Ligase is extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA.
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant
    
    Storage
    
    The enzyme is stable at -20°C for > 1 year in the supplied storage buffer.
    The enzyme tolerates at least four freeze-thaw cycles (-80°C) without loss of activity.
    
    Concentration
    
    250 kU: 5000 U/µl
    
    Activity
    
    T4 DNA Ligase can be completely inactivated by incubating at 70°C for 10 minutes.
    
    * For Research Use Only
- Applications
  - Application Description
    
    T4 DNA Ligase catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids.