Proteinase - Unspecific Endopeptidase

- Overview
  - Our proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.
    Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Pichia pastoris
    
    Type
    
    Recombinant; Nonspecific
    
    Storage
    
    The enzyme is stable upon storage at -20°C for >1 year. The proteinase is also stable upon storage at 4°C (>6 months) and room temperature (>4 weeks). The proteinase is stable at dilute concentrations (<0.1 mg/ml) in solutions.
    
    Note
    
    Tolerance to typical buffer additives:
    SDS: 0.2–1%
    Urea: 1–5 mM
    The enzyme is inhibited by general serine protease inhibitors, such as PMSF.
    
    Molecular Weight
    
    34 kDa
    
    Activity
    
    Highly active in temperature range of 25–40°C
    pH: 7–10
    
    * For Research Use Only
- Reference
  - 1. Moving from the bench towards a large scale, industrial platform process for adeno-associated viral vector purification.
    2. Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
    3. Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex.
    4. Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies.
    5. Multiplexed peroxidase-based electron microscopy labeling enables simultaneous visualization of multiple cell types.
    6. Near physiological spectral selectivity of cochlear optogenetics.
    7. Mining, analyzing, and integrating viral signals from metagenomic data.
    8. Structures of the Human PGD2 Receptor CRTH2 Reveal Novel Mechanisms for Ligand Recognition.
    9. Ultrafast optogenetic stimulation of the auditory pathway by targeting-optimized Chronos.
    10. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems.
    11. Global Representations of Goal-Directed Behavior in Distinct Cell Types of Mouse Neocortex.

Documents

Download Proteinase brochure

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