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Overview
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Cycloheximide is produced by the bacteria Streptomyces griseus. In eukaryotic organisms, it acts as an inhibitor of protein biosynthesis, interfering with protein elongation by binding to the E-site of ribosomes, after two elongation cycles. It has many in vitro applications, with the most popular being to determine a protein’s half-life. It is used as an antifungal in VTM. It does not appear to inhibit cell culture or viral replication at a concentration of 10ug/ml.
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Overview