HL-Exol - 3’-5’ Exonuclease

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      • Overview
        • HL-ExoI is a 3’-5’ exonuclease, specific for single stranded DNA. HL-ExoI is active at temperatures ≥25°C, and inactivated by 1 min incubation at 80°C, or 15 min at 60°C. Recombinantly produced in an E. coli strain expressing the HL-ExoI gene originating from an Arctic marine bacterium.
          Unit definition: One unit catalyses the release of 10 nmol of acid soluble nucleotides in 30 min at 25°C. Reaction buffer: 20 mM Tris-HCl pH 7.5 at 25°C, 200 mM NaCl, 20 mM MgCl2, 1 mM DTT, 0.01% Triton X-100, 2.5% glycerol and 3H-dATP-labelled DNA (25 ng/µl).

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      • Properties
        • Source
          E. coli
          The enzyme is purified to apparent homogeneity.
          The enzyme is stable at -20°C for > 3 years in the supplied storage buffer. The enzyme tolerates a minimum of five freeze-thaw cycles (- 80°C) without loss of activity

          * for research use only

      • Applications
        • Application Description
          3’-5’ exonuclease, specific for single stranded DNA
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