Login / Register

0

Comparison of inactivation properties of HL-ExoI and a commercial E.coli ExoI (EcExoI). After incubation for 10 min the nucleases were inactivated at 60°C for 15 min. The samples were then added a Fam labeled 20-mer and incubated for 30 min before visualization on a urea-PAGE gel.

Comparison of inactivation properties of HL-ExoI and a commercial E.coli ExoI (EcExoI). After incubation for 10 min the nucleases were inactivated at 60°C for 15 min. The samples were then added a Fam labeled 20-mer and incubated for 30 min before visualization on a urea-PAGE gel.

HL-Exol - 3’-5’ Exonuclease

HL-Exol - 3’-5’ Exonuclease

Catalog Number:

E001090413ARC

Mfr. No.:

70100-201/150

Price:

$216

Size:

Quantity:

Add to Cart:

- Overview
  - HL-ExoI is a 3’-5’ exonuclease, specific for single stranded DNA. HL-ExoI is active at temperatures ≥25°C, and inactivated by 1 min incubation at 80°C, or 15 min at 60°C. Recombinantly produced in an E. coli strain expressing the HL-ExoI gene originating from an Arctic marine bacterium.
    Unit definition: One unit catalyses the release of 10 nmol of acid soluble nucleotides in 30 min at 25°C. Reaction buffer: 20 mM Tris-HCl pH 7.5 at 25°C, 200 mM NaCl, 20 mM MgCl2, 1 mM DTT, 0.01% Triton X-100, 2.5% glycerol and 3H-dATP-labelled DNA (25 ng/µl).
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant
    
    Purification
    
    The enzyme is purified to apparent homogeneity.
    
    Storage
    
    The enzyme is stable at -20°C for > 3 years in the supplied storage buffer. The enzyme tolerates a minimum of five freeze-thaw cycles (- 80°C) without loss of activity
    
    * for research use only
- Applications
  - Application Description
    
    3’-5’ exonuclease, specific for single stranded DNA

Documents

Download HL Exol brochure

Note: If you don't receive our verification email, do the following:

Confirm that you entered your email address correctly.
Check if the email is in your spam or junk folder.
Or you may contact us at .

Products

Amerigo Scientific

Support

Contact Us

Copyright © Amerigo Scientific. All rights reserved.