SepSphere™ Small Molecule Alcohol Immobilization Kit

This personalized immobilization kit is designed for small molecule containing one hydroxyl (-OH) functional group. The customer supplies the small molecule. Using the kit components, the customer converts the hydroxyl group of the small molecule to an activated chloroacetyl group and the amine-modified agarose beads to a thiol-modified agarose, followed by the reaction of both components.

Note: Some small molecules may contain other functional groups that can interfere with this reaction. We recommend that customers first email information about their small molecule before placing an order for this kit so we can provide insight into the efficacy of this kit and potentially present alternative options.

• Key Features of this Immobilization Kit:
Offers a simple and easy way to label small molecules with a hydroxyl group
Includes all reagents and supplies needed for preparation
Uses agarose beads with an optimized amount of amine functional groups for loading to preserve the nature of the agarose beads
Minimum requirement for immobilization set-up
Easy preparation with air push mechanism for washing: 6 hr preparation time and less than 2 hr hands-on time

• Specification of the Solid Support
Functional groups: amine
Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
Particle size: 45-165 um
Mean bead size: 90 um
Ligand concentration: 10-30 umole per ml of settle beads

• Specification of the Immobilized Product
Immobilized chemistry: immobilized via amino agarose
Chemical stability: all commonly used aqueous buffers
pH Stability: long term: 3 to 9; short term: 2 to 10
Physical stability: negligible volume variation due to changes in pH or ionic strength

• Requirements for Small Molecules
Preferably >90% pure
Absence of other reactive functional groups, such as amine and thiol groups
For molecules containing a phenol group, multiple hydroxyl groups, or a secondary hydroxy group, please consult us prior to conducting the experiment

Please contact us at for specific academic pricing.

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