SepSphere™ Oligo Immobilization Kit via Carboxylic Acid

This oligo immobilization kit to work with amine-modified oligos. The user supplies the amine-modified oligos, which are readily available from many commercial oligo suppliers. The kit provides agarose beads derivatized with carboxylic acid functional groups (5-20 μmol COOH per mL of resin). Using the kit components, customers immobilize their amino containing oligo onto SepSphere™ agarose beads in one step following the standard protocol.

Key Features of this Immobilization Kit:
▪ Minimum requirement for immobilization set-up
▪ All reagents, buffers, and plasticwares are included
▪ Modestly activated carboxylic acid agarose
▪ One step immobilization with very mild reaction conditions
▪ Easy preparation with air push mechanism for washing: less than 1 hr hands-on time
▪ Stable linkage

Specification of the Solid Support
▪ Functional groups: carboxylic acid (COOH)
▪ Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
▪ Particle size: 45-165 um
▪ Mean bead size: 90 um
▪ Ligand concentration: 5-20 μmol COOH per mL agarose

Specification of the Immobilized Product
▪ Immobilization chemistry: immobilized via stable amide bond
▪ Chemical stability: stable
▪ pH Stability: stable

Requirement for oligo
▪ Preferable 5' C6 or 3' C6 amino modified oligo
▪ HPLC purified and lyophilized
▪ Total amount: 0.1-2 μmol
▪ Additional components of acid or other molecules containing primary or secondary amine groups and thiol should NOT be present
▪ Potential interfering compounds for labeling and conjugation reactions:
1. Thiols: e.g., DTT and mercaptoethanol
2. Primary and secondary amines: e.g., ammonium acetate, ammonium bicarbonate, ammonium tartrate, ammonium citrate, ammonium phosphate, AMPD [2-amino-2-methyl-1,3 propanediol], aminoguanidine bicarbonate salt, ethanol amine, Gly-gly, Tris buffer, piperidine buffers.

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