SepSphere™ Small Molecule Amine Immobilization Kit via Epoxide

This personalized immobilization kit is designed for small molecule containing amine functional group or phenol group (aromatic OH group). The user supplies the small molecule amine or phenol. Using the kit components, the customer immobilizes the small molecule onto SepSphere™ glyoxyl agarose in one step following the standard protocol.

This kit is custom made to order.
Some small molecules may contain other functional groups that can interfere with the reaction. We recommend that customers first email information about their small molecule before placing an order for this kit so we can provide insight into the efficacy of this kit and potentially present alternative options.

• Key Features of this Immobilization Kit:
Minimum requirement for immobilization set-up
All reagents, buffers, and plasticwares are included
Stable attachment
Easy preparation with air push mechanism for washing: less than 2 h hands-on time

• Specification of the Solid Support
Functional groups: Epoxide
Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
Particle size: 45-165 um
Mean bead size: 90 um
Ligand concentration: ~2 μmol epoxide per mL agarose

• Specification of the Immobilized Product
Immobilized chemistry: immobilized via ether (for phenol) or secondary amine (for amine)
Chemical stability: stable
pH Stability: stable

• Requirement for Small Molecule
Preferably >90% pure by C18 HPLC
Total amount: 1-10 mg
Stable at pH 10.0

Please contact us at for specific academic pricing.

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