Cycloheximide, CulturePure®

- Overview
  - Cycloheximide CulturePure® is a purified version (≥98% pure) of Cycloheximide that is free of toxic isomers. It is a glutarimide antibiotic and natural fungicide isolated from Streptomyces griseus and a protein synthesis inhibitor in eukaryotic cells. Cycloheximide was discovered by Alma Whiffen-Barksdale of Upjohn Company in 1946 and is now routinely used as a selection agent in several types of isolation media. In molecular biology, it can be used as an experimental tool to determine the half-life of a protein or to study protein synthesis and degradation. Cycloheximide can also be used in protein expression studies, translational profiling and ribosome profiling.
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    Background
    
    Cycloheximide binds to the ribosome and inhibits the eEF2-mediated translocation step in protein synthesis, thus blocking translational elongation.
- Properties
  - Categories
    
    CulturePure™ Products
    
    CAS Number
    
    66-81-9
    
    Molecular Formula
    
    C15H23NO4
    
    Molecular Weight
    
    281.35
    
    Appearance
    
    White or cream-colored powder
    
    Other Properties
    
    Source: Streptomyces griseus
    Loss on Drying: ≤1.0%
    Melting Point: 107-120°C
    Identification: HPLC
    Purity Level: (HPLC): ≥95.0%
    
    Storage
    
    ≤30°C
    
    * For research use only
- Applications
  - Application
    
    Spectrum; Microbiology Applications; Plant Biology Applications; Electrophoresis Applications; Cancer Applications
    
    Application Description
    
    Spectrum: Cycloheximide is used for fungi and yeast, including fungi found in brewing test media. It has lower activity against bacteria.
    
    Microbiology Applications: Cycoloheximide is routinely used as a selection agent in several types of isolation media: Columbia Blood Agar - Campylobacter Selective Supplement (Butzler) Dermasel Agar - Selective Supplement for dermatophyte fungi Campylobacter Agar - Campylobacter Selective Supplement (Preston) Listeria Selective Agar - Listeria Selective Supplement Listeria Enrichment Broth - Listeria Selective Enrichment Supplement Listeria Enrichment Broth - Modified Listeria Selective Enrichment Supplement STAA Agar - STAA Selective Supplement Legionella CYE Agar - Legionella GVPC Selective Supplement Campylobacter Agar - Campylobacter Selective Supplement (Karmali) Bolton Broth - Bolton Broth Selective SupplementRepresentative susceptibility data includes: Candida albicans 12.5 µg/ml Saccharomyces cerevisiae: 0.2 µg/ml Mycosphaerella graminicola: 5.62-100 µg/ml For additional Cycloheximide MIC data, please review the Antimicrobial Index.
    
    Plant Biology Applications: Cycloheximide is a commonly used lab reagent used in in vitro applications to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 uM have fungicidal effects (Schneider-Poetsch et al, 2009). The compound can be used as a plant growth regulator to stimulate ethylene production in leaves and fruit.
    
    Electrophoresis Applications: Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro. It inhibits the synthesis of proteins and macromolecules,and affects apoptosis in eukaryotes.
    
    Cancer Applications: Pretreatment with Cycloheximide followed by estrogen stimulation prevented the estrogen-induced changes in glucose metabolism in perfused breast cancer T47D clone 11 cells. This suggested that the estrogen stimulation requires synthesis of mRNA and protein (Neeman and Degani, 1989). In studying the “immune escape” of cancer cells, in human colorectal cancer cell line COLO 205 is normally resistant to TNF-alpha - a death inducing ligand. However, co-incubation TNF-alpha with Cycloheximide caused time-dependent cell death. In fact, authors found that Cycloheximide sensitizes cells to TNF-alpha-induced apoptosis (Pajak et al, 2005).