SepSphere™ Peptide Immobilization Kit via Pyridyl Disulfide

This personalized peptide immobilization kit is designed to work with thiol peptide. If your peptide does not have a Cys thiol group for immobilization, you can order a peptide with a Cys placed either at C-termini or N-termini. The kit provides agarose beads derivatized with pyridyl disulfide functional groups (5-20 μmol pyridyl disulfide per mL of resin). Using the kit components, customers immobilize their thiol containing peptide onto SepSphere™ agarose beads in one step following the standard protocol.

• Key Features of this Immobilization Kit:
Minimum requirement for immobilization set-up
All reagents, buffers, and plasticwares are included
Modestly activated pyridyl disulfide agarose
One step immobilization with very mild reaction conditions
Easy preparation with air push mechanism for washing: less than 1 hr hands-on time
Stable linkage (no leakage of the peptides from the resin under acid or base condition)

• Specification of the Solid Support
Functional groups: pyridyl disulfide
Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
Particle size: 45-165 um
Mean bead size: 90 um
Ligand concentration: 5-20 μmol pyridyl disulfide per mL agarose

• Specification of the Immobilized Product
Immobilization chemistry: immobilized via disulfide bond
Chemical stability: stable but can be cleaved to release the peptide by reducing reagent such as DTT, TCEP
pH Stability: stable

• Requirement for peptide
Cys containing peptide. If your peptide does not have a Cys, add a Cys at either C-termini or N-termini
HPLC purified and lyophilized, stored at -20 degree freezer all the time
Preferably >90% pure by C18 HPLC
Total amount: 1-20 mg
Does not contain any other reducing reagent such as DTT and mercaptoethanol

Please contact us at for specific academic pricing.

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