SepSphere™ Oligo Immobilization Kit for Phosphate DNA

This oligo immobilization kit is designed to work with oligo containing a 5' phosphate group. For PCR product you can order a primer with a 5'-phosphate group or use polynucleotide kinase to put the phosphate at the end. You can also order phosphate containing oligo directly from many commercial oligo suppliers.

The kit provides agarose beads derivatized with carboxylic acid functional groups (5-20 μmol COOH per mL of resin). Using the kit components, customers immobilize their phosphate containing oligo onto SepSphere™ agarose beads in two steps following the standard protocol.

• Key Features of this Immobilization Kit:
Minimum requirement for immobilization set-up
All reagents, buffers, and plasticwares are included
Modestly activated carboxylic acid agarose
One step immobilization with very mild reaction conditions
Easy preparation with air push mechanism for washing: less than 1 hr hands-on time
Stable linkage

• Specification of the Solid Support
Functional groups: COOH
Matrix: 4% beaded agarose (source: GE Life Sciences, Sepharose® 4B)
Particle size: 45-165 um
Mean bead size: 90 um
Ligand concentration: 5-20 μmol COOH per mL agarose

• Specification of the Immobilized Product
Immobilization chemistry: immobilized via stable amide bond
Chemical stability: stable
pH Stability: stable

• Requirement for oligo
>90% by gel electrophoresis
Total amount: 0.1-2 μmol
>10 nucleotide bases

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