PKmito DEEP RED

PKmito DEEP RED is a bright, non-phototoxic & non-toxic mitochondrial probe based on the PKmito™ dyes developed by Yang et al in the lab of Zhixing Chen1). PKmito DEEP RED labels mitochondria in live cells with very high specificity. The unique and unmatched feature of PKmito DEEP RED is its extremely low phototoxicity, due to the presence of the intramolecular triplet quencher cyclooctatetraene (COT) group. It allows to perform long term imaging of mitochondria without damaging them. PKmito DEEP RED accumulates in the mitochondrial inner membrane (IM) and can used to image mitochonridal cristae by STED or SIM superresolution microscopy. PKmito DEEP RED does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. PKmito DEEP RED enables multicolor imaging with SPY505, SPY555, SPY595, SPY620 or GFP. PKmito DEEP RED can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of PKmito DEEP RED (lyophilized).

Absorbance maximum λabs: 644 nm
Fluorescence maximum λfl: 670 nm
Works on fixed cells? No
Probe quantity: 100 stainings*
Fluorescence lifetime: 0.7 ns
STED depletion wavelength: 775 nm
Shipping: room temperature
Storage: -20°C

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