SiR700-Actin Kit

SiR700-actin is based on the far-red silicon rhodamine (SiR) fluorophore analogue SiR700 and the actin binding natural product jasplakinolide. SiR700-actin allows labeling of endogenous F-actin in live cells with high specificity and low background without the need for genetic manipulation or over-expression. The key features of SiR700-actin are i) far-red absorption and emission wavelengths, ii) cell permeability, iii) fluorogenic character, and iv) compatibility with super-resolution microscopy (e.g., STED & SIM). In addition, SiR700 actin can be used for wide-field and confocal fluorescent imaging in living cells. The emission in the far-red wavelength minimizes phototoxicity and sample autofluorescence. SiR700-actin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filter sets.

Probe quantity allows 35 – 140 staining experiments.*

Optical properties
λabs 689 nm
λEm 716 nm
εmax 1.0·105 mol-1·cm-1

MW 1265.6 g/mol
MF C73H88N8O10Si

*Based on the following conditions: 0.5 – 1 ml staining solution / staining experiment with 0.5 – 1 µM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

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