SPY555-DNA

SPY555-DNA is a bright & non toxic live cell nuclear stain based on our SPYTM dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY555-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission are similar to tetramethylrhodamine (TMR). SPY555-DNA enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SIR and GFP. SPY555-DNA can be imaged with standard TMR or Cy3 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Contains 1 vial of SPY555-DNA (lyophilized).

Absorbance maximum λabs: 555 nm
Fluorescence maximum λfl: 580 nm
Works on fixed cells? yes, PFA and methanol
Probe quantity: 100 stainings
Fluorescence lifetime: 2.4 ns
STED depletion wavelength: 660 or 775 nm
Shipping: room temperature
Storage: -20°C

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