HRP-Protein Conjugation Kit (LMW)

This personalized conjugation kit works with any small protein with a MW less than 20 KDa. Customer supplies its own unmodified protein. Using the kit components, the customer converts their protein to a thiol-protein, followed by reaction of the thiol-protein with activated HRP to generate the HRP-protein conjugates. The purification process typically provides the resulting HRP-protein at greater than 95% purity.

This kit provides materials to label one protein. Total amount of activated HRP included in a reaction: 2 mg.

• Requirement for protein:
Amount: 230 nmol (3.45 mg if the protein is 15 KDa) (Lower amount of protein is fine, but will require kit customization. Please contact us for the customization)
Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
Protein has some lysine residues

• Key Features of this HRP-Protein Conjugation Kit:
Use of heterobifunctional crosslinkers for direct labeling and conjugation in a two-step process
Better control over the conjugation process,
Limit self coupling and polymerization that often encountered when EDC or homobifunctional crosslinkers are used
High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity
Optimal maleimide groups per HRP for single label
Target 1-2 protein per HRP for a typical batch
A single purification step affords over 95% pure of HRP-protein conjugates
Fast preparation: less than 3 h hands-on time
All reagents included, from preparation to purification

Please contact us at for specific academic pricing.