HRP Oligo Conjugation Kit (Single Label with Thiol Oligo)

This kit (Cat#: CM53402) is upgraded to a new kit (Cat#: CM53408)
CM53407 has slightly different components and workflow in order to offer more flexibilities in selection of the oligo materials and improve the overall labeling efficiency.

This HRP-Oligo Conjugation kit works with protected thiol-modified oligos (disulfide form). The customer supplies the thiol-modified oligos containing 3’ C6 disulfide linker or 5’ C6 disulfide linker, which are readily available from many commercial oligo suppliers. Using the kit components, the customer reduces the disulfide bond of the oligo, followed by reaction of the thiol-oligo with activated horseradish peroxidase (HRP) to generate the HRP-oligo conjugates. The Q-column purification step typically provides the resulting HRP-oligo at greater than 85% purity.

This kit provides materials to label one (CM53402x1) or three (CM53402x3) oligo samples.

Shipping policy: heat-sensitive, choose overnight (ships Mon.-Thur.)

• Requirements for thiol-oligo
Amount: 5 nmol
The oligo is modified with 3’ C6 disulfide linker or 5’ C6 disulfide linker
Preferably, the disulfide-oligo should be HPLC purified and lyophilized

• Key Features of this HRP-Oligo Conjugation Kit
High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity
Optimal maleimide groups per HRP for single label of oligo: 1.2 for typical batch
A single purification step affords around 85-90% of single labeled HRP-oligo conjugates
Fast preparation in less than 1 hour of hands-on time
Easy purification in less than 30 minutes
No activity loss of the HRP after the conjugation and purification
All reagents included from preparation to purification

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