HRP-Protein Conjugation Kit (HMW)

This personalized HRP–protein conjugation kit is designed to work with any protein with a MW over 20KDa. The customer supplies their own unmodified protein. Using the kit components, the customer converts some of the surface amines of the protein to free thiol groups. The thiol-protein can then react with activated HRP to generate the HRP–protein conjugates. A subsequent capping step removes any unreacted thiols on the protein. A one step purification process typically provides the resulting HRP–Protein conjugates at greater than 90% content.

This kit provides materials to label one protein. Total amount of activated HRP included in a reaction: 2 mg.

• Requirement for protein:
Amount: 8 nmol (400 µg if the MW of protein is 50KDa)
Preferably >90% pure by gel electrophoresis or SEC HPLC analysis
Protein has some lysine residues

• Key Features of this HRP-Protein Conjugation Kit:
Use of heterobifunctional crosslinkers for direct labeling and conjugation in a two-step process
Better control over the conjugation process,
Limit self coupling and polymerization that often encountered when EDC or homobifunctional crosslinkers are used
High quality maleimide activated HRP for the conjugation: >99% purity and >200 units/mg protein activity
Target 1−3 HRP per protein (actual loading will depend on your protein)
≥90% HRP−Protein conjugates if the quality of the protein is at par
Preparation can be done in a day with less than 3 h hands-on time
All reagents included, from preparation to purification

Please contact us at for specific academic pricing.