Heat-Labile Salt Active Nuclease ( HL-SAN )

- Overview
  - This enzyme will allow for removal of nucleic acids in a traditional protein buffer regime – ie the protein is well protected while the nucleic acids are fully removed. Digests DNA and RNA in approximately a 10:1 ratio. Applications in removal of contaminating nucleic acids from purified enzyme/protein preps as well as inline digestion of nucleic acids in protein purifications. The digestion can take place at high salt conditions.
    
    Main advantages with Salt Active Nuclease
    • Non specific endonuclease
    • Optimum activity at high salt concentration (0.5 M NaCl)
    • Active at low temperatures (20% at 6ºC)
    • Broad pH range
    • Temperature stable
    
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    Nucleases
- Properties
  - Source
    
    Pichia pastoris
    
    Type
    
    Recombinant
    
    Activity
    
    HL-SAN is highly active in the temperature range 10–50°C. Optimal NaCl-concentration for activity is 0.5 M, working range is 0.25–1 M. Mg2+ (>1 mM) is required for activity. Working pH range is 7.5–9.5, optimal pH is 9.0.
    
    * For Research Use Only
- Reference
  - 1. Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection.
    2. A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry.
    3. Biochemical characterization of ParI, an orphan C5-DNA methyltransferase from Psychrobacter arcticus 273-4.
    4. Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome.
    5. Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida.

Documents

Download HL-SAN poster

Figure 2: Temperature and activity. HL-SAN has optimum activity at ~35°C, but works over a broad temperature range (20% activity at 10°C and 50°C). The activity of HL-SAN was tested in a 25 mM Tris-HCl buffer, pH 8.5 containing 5 mM MgCl2 and 0.5 M NaCl.

Figure 3: The effect of MgCl2 and MnCl2 concentration on the HL-SAN activity. The activity of HL-SAN was tested in a 25 mM Tris-HCl buffer, pH 8.5, 0.5 M NaCl and with varying concentrations of MgCl2 or MnCl2. The activity of the sample containing 5 mM MgCl2 was set to 100%.

Heat-Labile Salt Active Nuclease ( HL-SAN )

Heat-Labile Salt Active Nuclease ( HL-SAN )

More Details

Documents

Figure 2: Temperature and activity. HL-SAN has optimum activity at ~35°C, but works over a broad temperature range (20% activity at 10°C and 50°C). The activity of HL-SAN was tested in a 25 mM Tris-HCl buffer, pH 8.5 containing 5 mM MgCl2 and 0.5 M NaCl.

Figure 3: The effect of MgCl2 and MnCl2 concentration on the HL-SAN activity. The activity of HL-SAN was tested in a 25 mM Tris-HCl buffer, pH 8.5, 0.5 M NaCl and with varying concentrations of MgCl2 or MnCl2. The activity of the sample containing 5 mM MgCl2 was set to 100%.

Heat-Labile Salt Active Nuclease ( HL-SAN )

Heat-Labile Salt Active Nuclease ( HL-SAN )

More Details

Documents

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