Developed at ArcticZymes, first launched in 1993. Originates from the Arctic Shrimp (Pandalus borealis), from 2010 it has been produced as a recombinant version (rSAP). rSAP is better suited than native SAP (nSAP) for newer, more sensitive technologies.
Shrimp Alkaline Phosphatase (rSAP) has become one of today’s most-selling DNA modifying enzymes due to the added convenience through a complete heat inactivation. While most other alkaline phosphatases (from E. coli or Calf intestine) must be removed by extraction procedures, rSAP is completely inactivated after 15 minutes at 65°C. rSAP works well in common buffers without the requirement for other additions.
Main advantages with rSAP
• Very high specific activity
• 100% heat-inactivated at 65°C
• Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
• May be added directly to restriction enzyme digests
• No vector purification necessary
• Requires no supplemental zinc or other additives for activity
• Works directly in many different buffers
• Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis
Please contact us at for specific academic pricing.
StorageStable at -20ºC in storage buffer (25 mM Tris-HCl pH 7.6, 5 mM MgCl2, glycerol 50%). At room temperature, >95% activity remains after 90 days.ConcentrationMinimum 10 000 Units/ml, available up to 30 000 Units/ml.
* for research use only
ApplicationDephosphorylation; PCR product clean-up
Dephosphorylation of cloning vectors
1. Generating In Vivo Cloning Vectors for Parallel Cloning of Large Gene Clusters by Homologous Recombination.
Degradation of nucleotides before genotyping or sequencing
1. Comprehensive evaluation of coding region point mutations in microsatellite‐unstable colorectal cancer.
2. Molecular systematics of the subfamily Limenitidinae (Lepidoptera: Nymphalidae).
3. Detection and characterization of a rhabdovirus causing mortality in black bullhead catfish, Ameiurus melas.
4. Pellets of proof: First glimpse of the dietary composition of adult odonates as revealed by metabarcoding of feces.
5. Systematics and origin of moths in the subfamily Arctiinae (Lepidoptera, Erebidae) in the Neotropical region.
6. Medium-throughput SNP genotyping using mass spectrometry: multiplex SNP genotyping using the iPLEX® Gold assay.
7. Dried reagents for multiplex genotyping by tag-array minisequencing to be used in microfluidic devices.
8. Highly multiplexed genotyping of thiopurine s-methyltransferase variants using MALD-TOF mass spectrometry: reliable genotyping in different ethnic groups.
9. Genotyping SNPs using a UV-photocleavable oligonucleotide in MALDI-TOF MS.
10. A gel-free SNP genotyping method: bioluminometric assay coupled with modified primer extension reactions (BAMPER) directly from double-stranded PCR products.
11. G2 checkpoint in uterine cervical cancer with HPV 16 E6 according to p53 polymorphism and its screening value.
12. A novel procedure for efficient genotyping of single nucleotide polymorphisms.
1. Autophosphorylation activates Dictyostelium myosin II heavy chain kinase A by providing a ligand for an allosteric binding site in the alpha-kinase domain.
2. A diurnally regulated dehydrin from Avicennia marina that shows nucleo-cytoplasmic localization and is phosphorylated by Casein kinase II in vitro.
SAP for quantification
1. Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters.
2. A comparison of enzymatic digestion for the quantitation of an oligonucleotide by liquid chromatography-isotope dilution mass spectrometry.
SAP for pyrosequensing
1. Method enabling pyrosequencing on double-stranded DNA.
1. Sensitive electrochemical assay of alkaline phosphatase activity based on TdT-mediated hemin/G-quadruplex DNAzyme nanowires for signal amplification. (ScienceDirect pay service).
2. Ligand-binding and metal-exchange crystallographic studies on shrimp alkaline phosphatase.
3. Thermolabile alkaline phosphatase from Northern shrimp (Pandalus borealis): protein and cDNA sequence analyses.