Florfenicol Amine

76639-93-5

C10H14FNO3S

247.3

White solid

soluble in ethanol, methanol, DMF or DMSO. Limited water solubility.

-20°C

Microbiology Applications: Residues can be detected via liquid chromatography: high performance liquid chromatography (HPLC) ultra-high performance liquid chromatography (UHPLC) ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) (ie food samples; chicken muscle) reversed-phase high-performance liquid chromatography with fluorescence detection (RP-HPLC-FLD)(ie eggs) Residues can be detected by gas chromatography: Gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS) (ie poultry, porcine muscle) Gas chromatography with electron capture detection (ie shrimp)
Plant Biology Applications: Breast cancer resistance protein (BCRP) is one of the most important ATP-binding cassette superfamily transporters. Although it was discovered in cancer cells and could recognize anticancer agents, it is also expressed in other normal tissues of animals such as the apical membrane of enterocytes, hepatocytes and renal tubular cells where they can limit GI absorption. The US FDA has recommended to include whether a candidate compound is a substrate and/or inhibitor of BCRP during its development for those compounds with a net efflux ratio > 2, since it could be considered as a potential substrate of an efflux pump (Ref 9). Researchers found that Florfenicol is a substrate of BCRP as indicated by the bidirectional transport assay in Madin-Darby canine kidney (MDCK) and MDCK-chABcg2 cells (Liu et al, 2018).