Florfenicol

73231-34-2

C12H14Cl2FNO4S

358.21

White crystalline powder

Soluble in DMSO and DMF. Sparingly soluble in aqueous buffers.

Source: Derivative of Thiamphenicol
PH: 4.5-6.5
Assay: (On Dried Basis): ≥98.0%
Loss on Drying: ≤0.5%
Melting Point: 152-156°C
Optical Rotation: -16° to -19°

2-8°C

Spectrum: Florfenicol is a broad-spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria, especially those causing bovine respiratory infections such as bovine respiratory disease (Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni). It can also be used for the bacteria causing enteric septicemia in catfish.

Microbiology Applications: Florfenicol is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
Haemophilus parasuis 0.24 µg/mL – 4 µg/mL
Escherichia coli 0.5 µg/mL – 128 µg/mL
The first Florfenicol resistance gene (pp-flo) was found in a fish pathogen. Plasmid-mediated Florfenicol resistance is encoded by the floR gene in E. coli (Cloeckaert et al, 2000).

Eukaryotic Cell Culture Applications: Florfenicol was found to show immunosuppressive activity on mouse splenocytes that were harvested to assess lymphocyte proliferation of CD3+T and CD19+B lymphocytes. The compound suppressed splenocyte proliferation, demonstrating that it could suppress humoral and cellular immune responses in mice (Shuang et al, 2011).

Cancer Applications: Florfenicol is a substrate of Breast Cancer Resistance Protein (BCRP) as indicated by the bidirectional transport assay in Madin-Darby canine kidney (MDCK) and MDCK-chABcg2 cells. BCRP is one of the most important ATP-binding cassette superfamily transporters. Although it was discovered in cancer, it is also expressed in other normal animal tissues such as the apical membrane of enterocytes, hepatocytes and renal tubular cells where they can limit GI absorption (Liu et al, 2018).