PKmito RED

PKmito RED is a bright, non-phototoxic & non-toxic mitochondrial probe based on the PKmito™ dyes developed by by Yang et al in the lab of Zhixing Chen1). PKmito RED labels mitochondria in live cells with very high specificity. The unique and unmatched feature of PKmito RED is its extremely low phototoxicity, due to the presence of the intramolecular triplet quencher cyclooctatetraene (COT) group. It allows to perform long term imaging of mitochondria without damaging them. PKmito RED accumulates in the mitochondrial inner membrane (IM) and can used to image mitochonridal cristae by STED or SIM superresolution microscopy. PKmito RED does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. PKmito RED enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR or GFP. PKmito RED can be imaged with a standard TMR or Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of PKmito RED (lyophilized).

Absorbance maximum λabs: 549 nm
Fluorescence maximum λfl: 569 nm
Works on fixed cells? No
Probe quantity: 100 stainings*
Fluorescence lifetime: 0.8 ns
STED depletion wavelength: 660 nm
Shipping: room temperature
Storage: -20°C

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