Cdc42 protein: GSt tagged: human wid type

- Overview
  - Product Uses Include:
    Cdc42 biochemistry
    Cdc42 GTPase assays
    Cdc42 nucleotide exchange assays
    Cdc42 binding studies
    
    Material
    The human Cdc42 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder. When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
    The recombinant protein is 50 kDa, consisting of the 22 kDa Cdc42 protein plus a 28 kDa GST tag in the amino-terminus.
    
    Please contact us at for specific academic pricing.
- Properties
  - Purification
    
    Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. GST-Cdc42 samples are >90% pure.
    
    * For Research Use Only
- Reference
  - Cheng, Zhi Jie et al. Co-regulation of caveolar and Cdc42-dependent fluid phase endocytosis by phosphocaveolin-1. Journal of Biological Chemistry. 2010
    Zhang, Xiao Feng et al. Rho-Dependent Contractile Responses in the Neuronal Growth Cone Are Independent of Classical Peripheral Retrograde Actin Flow. Neuron. 2003
    Lesser, C. F. et al. Expression of microbial virulence proteins in Saccharomyces cerevisiae models mammalian infection. The EMBO Journal. 2001
    Slater, S. J. et al. Interaction of Protein Kinase C Isozymes with Rho GTPases†. Biochemistry. 2001

Datasheet

MSDS

Cdc42 protein: GSt tagged: human wid type

Cdc42 protein: GSt tagged: human wid type

We Also Recommend

CENP-E kinesin motor domain protein (KIF10): GST tagged: Homo sapiens recombinant

Rac1 protein: His tagged: human constitutively active

Signal-Seeker™ SUMOylation 2/3 Detection Kit (30 assay)