PepMute™ siRNA Transfection Reagent

PepMute™ siRNA Transfection Reagent

Catalog Number:
TR02401829SIG
Mfr. No.:
SL100566
Price:
$1,260
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      • Overview
        • PepMute™ siRNA Transfection Reagent, formulated from simulation of virus cell penetrating peptides (CPPs), is a total novel siRNA delivery tool which provides more than 95% silencing efficiency at 1 nM siRNA in variety of mammalian cells. With our proprietary peptide simulation technology (PST), hundreds of viral CPPs were simulated, synthesized and screened for gene delivery efficacy in variety of mammalian cells. PepMute™ reagent was then identified and validated as an exceptionally efficient vector for condensing and transfecting short (under 100 bp) single or double stranded nucleic acid s such as siRNA, miRNA mimics and DNA oligoes to wide spectrum of mammalian cells.

          - Excellent silencing at final 1.0 nM siRNA
          - Over 95% gene silencing in a wide variety of cells
          - One tube reaction with easy standard protocol
          - Compatible with serum and antibiotics
          - Protocols adapted to HTS
          - Very low cytotoxicity
          - Very affordable

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          In Vitro siRNA Transfection Reagents
          Storage
          Store at 4 °C for PepMute ™ Reagent and RT for PepMute™ Transfection Buffer (5x ). If stored properly, the product is stable for 12 months or longer.
          Note
          PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium. Culturing your cells with other cell media (supplemented with 10% FBS and antibiotics) will basically eliminate the transfection toxicity. If you find toxicity and high cell death, it may be because DMEM base medium supplemented with 10% FBS and antibiotics was used to culture the cells. To remove the transfection toxicity, DMEM can be replaced with DMEM/F12 or RPMI 1640 as base medium. Although we haven't figured out why, it does minimize toxicity. Please try it!

          * This product is for laboratory research ONLY and not for diagnostic use.

      • Applications
        • Application Description
          - siRNA miRNA mimics or mRNA transfection
          - DNA/siRNA co-transfection
          - DNA oligoes transfection

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