PepMute™ Plus siRNA Transfection Reagent

PepMute™ Plus siRNA Transfection Reagent

Catalog Number:
TR02401827SIG
Mfr. No.:
SL100571
Price:
$2,080
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      • Overview
        • PepMute™ Plus siRNA & DNA Transfection Reagent is an upgraded version of PepMute™ reagent (Cat # SL100566) by crosslinking pre-screened hydrophobic amino acids to its backbone. With our proprietary pH Dependent Conformational Change ( PDCC) technology, the transfection reagent which contains 36 amino acids long's peptide backbone was formulated by addition of pre-screened hydrophobic amino acids to all amine groups, conferring PepMute™ Plus reagent an ability to self-assembly during formation of transfection complex and making PepMute™ reagent a versatile and most powerful gene delivery tool which provides more than 95% silencing efficiency at 1 nM siRNA in variety of mammalian cells. PepMute™ Plus Reagent have been validated to effectively and reproducibly transfect single DNA or siRNA or co-transfect DNA/siRNA to variety of mammalian cells.

          - Effective knockdown (95%) with only 0.5 pmol siRNA (1.0 nM siRNA in 24-well plate)
          - Formulated for hard-to-transfect mammalian cells
          - One tube reaction and easy transfection protocol
          - Best for broad spectrum especially hard-to-transfect mammalian cells
          - Very low cytotoxicity

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          In Vitro siRNA Transfection Reagents
          Other Properties
          Content:
          -PepMute™ Plus Transfection Reagent, 1.0 mL, sufficient for ~1000 reactions based on transfecting 2.5 pmols siRNA in 24-well plate
          -PepMute™ Plus Transfection Buffer(5x ), formulated for maximal transfection efficiency, 8.0 mL
          Storage
          Store at 4 °C for PepMute™ Plus Reagent and RT for PepMute™ Transfection Buffer (5x ). If stored properly, the product is stable for 12 months or longer.
          Note
          PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium. Culturing your cells with other cell media (supplemented with 10% FBS and antibiotics) will basically eliminate the transfection toxicity. If you find toxicity and high cell death, it may be because DMEM base medium supplemented with 10% FBS and antibiotics was used to culture the cells. To remove the transfection toxicity, DMEM can be replaced with DMEM/F12 or RPMI 1640 as base medium. Although we haven't figured out why, it does minimize toxicity. Please try it!

          * This product is for laboratory research ONLY and not for diagnostic use.

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