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Figure 1. Immunohistochemical identification of PACAP Receptor 1 in mouse spinal cord dorsal horn. Brain sections were dewaxed, microwaved in citric acid, and incubated with anti-PAC1 (PACAP receptor 1) antibody (7TM0370MN-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, PAC1 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 2. Immunocytochemical identification of PACAP Receptor 1 in HEK293 cells. HEK293 cells stably expressing the PACAP Receptor 1 (PAC1) were either not exposed or exposed to 1 μM PACAP (pituitary adenylate cyclase-activating peptide)) for 30 min and immunocytochemically stained with anti-PAC1 (PACAP receptor 1) antibody (7TM0370MN-IC) at a dilution of 1:200. Note, PAC1 receptors were confined to the plasma membrane in untreated cells (0 min). PAC1 receptors were seen in perinuclear clusters of vesicles after 30 min PACAP exposure.

Figure 3. Validation of the PACAP Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the mouse PACAP Receptor 1 (PAC1) were lysed and immunoblotted with the phosphorylation-independent anti-PAC1 antibody (7TM0370MN-IC) at a dilution of 1:1000.

mPAC1 ( IHC-grade ), PACAP Receptor 1 Polyclonal Antibody ( Mouse )

mPAC1 ( IHC-grade ), PACAP Receptor 1 Polyclonal Antibody ( Mouse )

Catalog Number:

A001456005TMA

Mfr. No.:

7TM0370MN-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The mouse PAC1 antibody is directed against the distal end of the carboxyl-terminal tail of mouse and rat PACAP receptor 1. It can be used to detect total PAC1 receptors in Western blots independent of phosphorylation. The mouse PAC1 antibody can also be used to isolate and enrich PAC1 receptors from cell and tissue lysates. It also detects PAC1 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  mouse. rat
  
  Immunogen
  
  A synthetic peptide presents distal end of carboxyl-terminal tail of mouse and rat PAC1
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  PAC1, PACAP Receptor 1, Pituitary Adenylate Cyclase Activating Polypeptide 1 Receptor (UniProt:P70205 (mouse), P32215 (rat))
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Liu NJ, Schnell SA, Schulz S, Wessendorf MW, Gintzler AR. Regulation of spinal dynorphin 1-17 release by endogenous pituitary adenylyl cyclase-activating polypeptide in the male rat: relevance of excitation via disinhibition. J Pharmacol Exp Ther. 2011 Feb;336(2):328-35. doi: 10.1124/jpet.110.173039. Epub 2010 Oct 25. PubMed PMID: 20974701; PubMed Central PMCID: PMC3033715.

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