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Figure 1. Immunohistochemical identification of FFA4 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA4 antibody (7TM0127N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA4 receptors were detected at the plasma membrane of cells in human spleen.

Figure 2. Detection of non-phosphorylated human FFA4 Receptor. Flp-In TREx 293 harbouring human FFA4-eYFP induced to express the receptor construct were treated with vehicle or 10 µM TUG-891 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with anti-non-phospho-FFA4 (7TM0127N-IC) at a dilution of 1:1000 (upper panel) or anti-GFP 1:10,000 to confirm expression and equal loading of the gel (lower panel).

FFA4 ( IHC-grade ), FFA4 Receptor Polyclonal Antibody

FFA4 ( IHC-grade ), FFA4 Receptor Polyclonal Antibody

Catalog Number:

A001456006TMA

Mfr. No.:

7TM0127N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The FFA4 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human FFA4. It can be used to detect total FFA4 receptors in Western blots independent of phosphorylation. The FFA4 antibody can also be used to detect FFA4 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide presents the carboxyl-terminal tail of human FFA4.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  PGPR4, Omega-3 fatty acid Receptor 1, O3FAR1 (UniProt:Q5NUL3)
  
  * This product is intended for research use only.

Applications

Application

WB

Application	Dilutions
Western Blot (WB)	1:1000

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