MGIEasy Fast RNA Library Prep Set

The MGIEasy Fast RNA Library Prep Set is a specifically designed RNA rapid library prep product for DNBSEQTM sequencers. The library prep set is optimized to convert 10ng~1μg of total RNA into a DNA library for gene expression profiling, transcriptome analysis, or detection of pathogenic microorganisms in high-throughput RNA sequencing on DNBSEQTM sequencers.

The library prep set is optimized with high quality enzyme and buffer system to combine cDNA second strand synthesis and end repair steps, which greatly shortens the time of library construction. The second strand synthesis is equipped with two kinds of buffers that can be used to prepare a common or directional library according to your needs. dTTP is replaced with dUTP in the strand-specific second strand synthesis buffer so that dUTP is incorporated into the second strand of cDNA. The high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, which achieves strand specificity.

Features
• Fast library prep workflow
Combine the cDNA second strand synthesis, end repair and dA-tailing into one step, greatly shorten the time of library prep time and minimize hands-on time.
• Flexible library perp with two library types
Provide two kinds of cDNA second strand synthesis buffer, support to prepare a common or directional library.
• Excellent performance
High uniformity coverage across transcripts, accurate detection of strand orientation, high concordance of gene expression.
• Wide range of total RNA input and multiples sample types
Compatible with a range of total RNA input (10ng~1ug) and multiples sample types, including low-quality RNA, FFPE RNA.
• Flexible Solutions
Adapt to various RNA enrichment methods, sequencing read lengths, two DNB method (universal circularization or OneStep DNB) to meet the needs of different sample types.

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