MGIEasy Fast PCR-FREE FS Library Prep Set V2.0

The MGIEasy Fast PCR-FREE FS Library Prep Set V2.0 is designed to prepare WGS PCR-Free library for DNBSEQTM sequencers. This library prep set is optimized to convert 25ng~900ng genomic DNA (gDNA) into a special library and uses high quality fast fragmentase to combine the fragmentation, end-repair and add A into one step, to simplify the preparation process and significantly shorten the duration of DNA library preparation. Fast PCR-FREE combined with MGI DNBSEQTM sequencer, is truly PCR-free sequencing with no amplification errors accumulation.

The PCR-free method and optimized library prep make it perform well in genome coverage uniformity and mutation detection precision and sensitivity. The set combined with the MGISP-100 and MGISP-960, could provide automatic library preparation workflows. This library prep set is suitable for WGS with fast, easy-to-use, and excellent performance and is the preferred recommendation for the large genome sequencing project.

Features
• Fast library prep workflow
Combine the DNA fragmentation, end repair and add A into one step, significantly reducing the library prep time in 1.3 hours.
• Flexible workflow to accommodate variations in sample type, DNA input amount, application
Compatible with 25ng~900ng DNA input, supporting variety sample types, including blood, saliva, oral swabs and virus long amplicons, suitable for high and low depth WGS of various species, as well as microbial sequencing and pathogen amplicon detection.
• High uniform and consistent insert sizes across a wide DNA input range and sample types
Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the high uniform and consistent insert sizes.
• Excellent performance
High uniform genome coverage, better coverage of GC-rich regions. Outstanding mutation detection precision and sensitivity performance.
• Simple and automated workflow
Minimal operation steps, simple and easy to use. Compatible with automatic sample preparation systems to provide automated workflow.

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