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Figure 1. Validation of the FFA2 in transfected HEK293 cells. Flp-In TREx HEK293 cells were induced to express human FFA2 DREADD-eYFP were treated with vehicle or 100 nM sorbate for 5 min. FFA2 receptors were enriched using a GFP tra and immunoblotted with the phosphorylation-independent anti-FFA2 antibody (7TM0226N-IC) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of FFA2 Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA2 (FFA2 Receptor) antibody (7TM0226N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA2 receptors were detected in a subset of pacreatic islet cells and at th eplasma membrane of exocrine pancreas cells.

Figure 3. Immunohistochemical identification of FFA2 Receptor in liver. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA2 (FFA2 Receptor) antibody (7TM0226N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA2 receptors were detected at the plasma membrane of hepatocytes.

Figure 4. Immunohistochemical identification of FFA2 Receptor in colon carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-FFA2 (FFA2 Receptor) antibody (7TM0226N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, FFA2 receptors were detected at the plasma membrane of colon carcinoma cells.

FFA2 ( non-phospho-FFA2 Polyclonal Antibody )

FFA2 ( non-phospho-FFA2 Polyclonal Antibody )

Catalog Number:

A001455924TMA

Mfr. No.:

7TM0226N

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The non-phospho-FFA2 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human FFA2. It can be used to detect total FFA2 receptors in Western blots independent of phosphorylation. The non-phospho-FFA2 antibody can also be used to isolate and enrich FFA2 receptors from cell and tissue lysates. It also detects FFA2 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide presents the carboxyl-terminal tail of human FFA2.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  FFAR2 (UniProt:O15552)
  
  * This product is intended for research use only.

Applications

Application

WB; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100

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