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Figure 1. Validation of the Dopamine Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Dopamine Receptor 1 (D1) were lysed and immunoblotted with the phosphorylation-independent anti-D1 antibody (7TM0214N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Dopamine Receptor 1 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D1 (Dopamine Receptor 1) antibody (7TM0214N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunocytochemical identification of Dopamine Receptor 1 in transfected HEK293 cells. HEK293 cells stably expressing the Dopamine Receptor 1 (D1) were either not exposed or exposed to 100 nM Dopamine for 30 min and immunocytochemically stained with anti-D1 antibody (7TM0214N-IC) at a dilution of 1:200. Note, D1 receptors were confined to the plasma membrane in untreated cells (MOCK). D1 receptors were seen in perinuclear clusters of vesicles after 30 min Dopamine exposure.

Figure 4. Immunohistochemical identification of Dopamine Receptor 1 and Dopamine Receptor 2 in striatum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-D1 (Dopamine Receptor 1) antibody (7TM0214N-IC) at a dilution of 1:100 (left panel) or incubated with anti-D2 (Dopamine Receptor 2) antibody (7TM0215N-IC) at a dilution of 1:100 (right panel). Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin

D1 ( non-phospho-Dopamine Receptor 1 Polyclonal Antibody )

D1 ( non-phospho-Dopamine Receptor 1 Polyclonal Antibody )

Catalog Number:

A001455927TMA

Mfr. No.:

7TM0214N

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The non-phospho-D1 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human D1. It can be used to detect total D1 receptors in Western blots independent of phosphorylation. The non-phospho-D1 antibody can also be used to isolate and enrich D1 receptors from cell and tissue lysates. It also detects D1 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide presents the carboxyl-terminal tail of human D1.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  D1, DADR, Dopamine Receptor 1, Dopamine-1A Receptor 1 (UniProt:P21728)
  
  * This product is intended for research use only.

Applications

Application

WB; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100

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