Anti-profilin: rabbit polyclonal

- Overview
  - Profilin antibody is provided as an affinity purified rabbit polyclonal antibody. The antibody has been raised against purified human profilin and it is predicted to have broad species cross reactivity. The positive control protein consists of an extract of bovine thymus that identifies a characteristic profilin band at 14 kDa. The control protein (bovine thymus extract, Cat. # EXT03) is supplied as a lyophilized powder; this should be reconstituted in 500 μl of cold Milli-Q water to 2 mg/ml. After addition of water the prot
    ein should be left at room temperature for 5 minutes with occasional pipetting up and down to make sure that the protein has completely dissolved into solution, it can then be stored at -20°C. We recommend running 10 μg of positive control protein in Western blots.
    
    Materials
    The profilin antibody (50 μg) is supplied as a lyophilized powder. It is recommended to resuspend the powder in 200 μl of 30% glycerol/water. This gives a concentration of 250 μg/ml in PBS plus 30% glycerol with 0.01% chlorhexidine for preservative.
    
    Please contact us at for specific academic pricing.
- Properties
  - * For Research Use Only
- Reference
  - Romero, Ana M. et al. Chronic alcohol alters dendritic spine development in neurons in primary culture. Neurotoxicity research. 2013
    Osiak, Anna Eleonor et al. Subconfluent endothelial cells form podosomes downstream of cytokine and RhoGTPase signaling. Experimental cell research. 2005
    Hernández-Deviez, Delia J. et al. ARNO and ARF6 regulate axonal elongation and branching through downstream activation of phosphatidylinositol 4-phosphate 5-kinase alpha. Molecular biology of the cell. 2004
    Innocenti, Metello et al. Abi1 is essential for the formation and activation of a WAVE2 signalling complex. Nature cell biology. 2004
    Tang, Dale D. et al. Role of Crk-Associated Substrate in the Regulation of Vascular Smooth Muscle Contraction. Hypertension. 2003
    Tang, Dale D. et al. Downregulation of profilin with antisense oligodeoxynucleotides inhibits force development during stimulation of smooth muscle. American journal of physiology. Heart and circulatory physiology. 2003

Datasheet

MSDS

Anti-profilin: rabbit polyclonal

Anti-profilin: rabbit polyclonal

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