Vav1 GEF Protein (DHPHC1 Exchange Domain, Y174D mutant, aa168-522, 6xHis tag)

Product Uses
Vav1 protein is a guanine exchange factor with selectivity for Rac1, which mediates Rac1 activation under a variety of conditions and has been associated with multiple diseases for example autoimmune deficiency and esophageal squamous cell carcinoma (reviewed in 1, 2, 3).
Study inhibitors of Vav1 GTP/GDP exchange activity
Identification of Vav1 DHPHC1 domain binding proteins
Study of Vav1 GEF activity with different GTPases

Material
The DHPHC1 domain of human Vav1 protein has been produced in a bacterial expression system. It contains a mutated amino-acid that mimics tyrosine phosphorylation which is required for interaction with Rac1 (Y174D mutant). It is also 6xHis tagged at its amino terminus for purification purposes. The accession number is AAH13361. The molecular weight of GE05 is approximately 41 kDa. The Vav1 protein is supplied as a white lyophilized powder. Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gradient gel. Vav1 DHPHC1 protein was determined to be approximately 80% pure.

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