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Overview
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X-rhodamine microtubules formed from X-rhodamine labeled tubulin.
Product Uses Include:
Laser based applications
Monitoring microtubule dynamcs in living cells
Speckle microscopy
Formation of fluorescent microtubules
Microscopy studies of MAP and microtubule associated motor activities
Nanotechnology
Material
Porcine brain tubulin (>99% pure, see Cat. #T240) has been modified to contain covalently linked X-rhodamine at random surface lysines. An activated ester of X-rhodamine was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficient when protein bound is 66,000M-1cm-1). Final labeling stoichiometry is 1-2 dyes per tubulin heterodimer. X-rhodamine labeled tubulin can be detected using a filter set of 540-560 nm excitation and 610-630 emission. X-rhodamine tubulin is in a versatile, stable and easily shipped format. It is ready for micro-injection or in vitro polymerization. We also offer AMCA (Cat. # TL440M), HiLyte Fluor™ 488TM (Cat. # TL488M), rhodamine (Cat. # TL590M) and HiLyte Fluor™ 647TM (Cat. # TL670M) labeled tubulins of the same quality.Please contact us at for specific academic pricing.
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- Properties
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Overview