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Overview
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HiLyte Fluor™ 488 labeled microtubules formed from HiLyte Fluor™ 488 labeled tubulin.
Product Uses Include:
Laser based applications
Monitoring microtubule dynamcs in living cells
Speckle microscopy
Formation of fluorescent microtubules
Microscopy studies of MAP and microtubule associated motor activities
Nanotechnology
Material
Porcine brain tubulin (>99% pure, see Cat. # T240) has been modified to contain covalently linked HiLyte Fluor™ 488 (HiLyte Fluor is a trademark of Anaspec Inc, CA) at random surface lysines. An activated ester of HiLyte Fluor™ 488 was used to label the protein. Labeling stoichiometry was determined by spectroscopic measurement of protein and dye concentrations (dye extinction coefficient when protein bound is 76,000M-1cm-1). Final labeling stoichiometry is 1-2 dyes per tubulin heterodimer. HiLyte Fluor™ 488 labeled tubulin can be detected using a filter set of 440-460 nm excitation and 500-520 emission. HiLyte Fluor™ 488 tubulin is in a versatile, stable and easily shipped format. It is ready for micro-injection or in vitro polymerization. We also offer AMCA (Cat. # TL440M), rhodamine (Cat. # TL590M), X-rhodamine (Cat. # TL620M) and HiLyte Fluor™ 647 (Cat. # TL670M) labeled tubulins of the same quality.Please contact us at for specific academic pricing.
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- Properties
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Overview