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Overview
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Product Uses Include:
Screening compounds for effects on tubulin polymerization activity.
Screening proteins for effects on tubulin polymerization activity.
Teaching aid for undergraduate/graduate class in pharmacology.
Economical approach to HTS with tubulin.
Introduction
This assay is based on an adaptation of the original method of Shelanski et al. and Lee et al. (1,2), which demonstrated that light is scattered by microtubules to an extent that is proportional to the concentration of microtubule polymer. The resulting polymerization curve is representative of the three phases of microtubule polymerization, namely nucleation, growth and steady state equilibrium.
This kit contains "HTS tubulin", which is >97% pure (Cat. # HTS03). The same assay is also available with our highest purity tubulin (>99% pure, Cat. # T240) and is sold under Cat. # BK006P. BK004P provides an economical alternative to the higher purity BK006P assay and can be a good choice for large primary screens. We also provide a fluorescence based tubulin polymerization assay in miniaturized format (Cat. # BK011P), which is also ideal for high throughput screening.
References
Shelanski, M. L., Gaskin, F. and Cantor, C. R. (1973). Microtubule assembly in the absence of added nucleotides. Proc. Natl. Acad. Sci. U.S.A. 70, 765-768.
Lee, J. C. and Timasheff, S. N. (1977). In vitro reconstitution of calf brain microtubules: effects of solution variable. Biochemistry, 16, 1754-1762.Please contact us at for specific academic pricing.
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Overview