TRANSCRIPTME Reverse Transcriptase (RT32)

- Overview
  - TRANSCRIPTME is a modified, recombinant form of the Reverse Transcriptase from the Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. TRANSCRIPTME synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.
    
    TRANSCRIPTME Reverse Transcriptase has increased thermal stability, which allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ –› 5’ exonuclease or RNase H activity, which improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNA synthesis up to 7 kb long.
    
    Features and advantages
    • High yields of full-length cDNA synthesis (up to 7 kb long)
    • RNA and DNA-dependent DNA polymerase activities
    • Increased sensitivity in RT-qPCR and RT-PCR assays
    • Starting material: 10 pg – 5 µg of total RNA or 10 pg – 500 ng of mRNA
    • Optimal reaction temperature: 50°C
    • Increased thermostability
    • No RNase H or 3’–› 5’ exonuclease activities
    • Suitable for the amplification of difficult RNA templates
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Enzymes
- Applications
  - Application
    
    cDNA synthesis; cDNA library construction; RNA analysis
    
    Application Description
    
    TRANSCRIPTME is a modified, recombinant form of the Reverse Transcriptase from Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. TRANSCRIPTME Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.
- Reference
  - In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections
    Hybridization-based in situ sequencing (HybISS) for spatially resolved transcriptomics in human and mouse brain tissue
    Expression of Toll‐like receptors and costimulatory molecules in splenic B cells in a normal and abortion‐prone murine pregnancy model
    A comprehensive transcriptome analysis of skeletal muscles in two Polish pig breeds differing in fat and meat quality traits
    Visualization of tumor heterogeneity by in situ padlock probe technology in colorectal cancer
    Oligonucleotide gap-fill ligation for mutation detection and sequencing in situ