TRANSCRIPTME LYO Reverse Transcriptase (RT32L)

TRANSCRIPTME LYO Reverse Transcriptase is a freeze-dried form of a modified Reverse Transcriptase originated from Moloney Murine Leukemia Virus (M-MuLV) expressed in Escherichia coli. TRANSCRIPTME LYO Reverse Transcriptase synthesizes a complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA (ssDNA) as a template.

TRANSCRIPTME LYO Reverse Transcriptase has increased thermal stability, that allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ –› 5’exonuclease and reduced RNase H activity, that improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNA synthesis up to 7 kb long.

Features and advantages
• Increased stability (transport at ambient temperature)
• High yields of full-length cDNA synthesis (up to 7 kb long)
• RNA- and DNA-dependent DNA polymerase activity
• Increased sensitivity in RT-LAMP, RT-qPCR, and RT-PCR assays
• Starting material: 10 pg – 5 μg of total RNA or 10 pg – 500 ng of mRNA
• Optimal reaction temperature: 50°C
• Increased thermostability
• No 3’ –› 5’ exonuclease activity
• Reduced RNase H activity
Suitable for amplification of difficult RNA templates

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