SPY620-BG Substrate

SPY620-BG is the benzylguanine derivative of our SPY620 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY620-BG can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has a structure similar to MaP618 fluorophore the name used in its original publication in Nature Chemistry1).
Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.

*SNAP-tag™ and SNAP-Cell® are registered trademarks of New England Biolabs, Inc.

Absorbance maximum λabs: 619 nm
Fluorescence maximum λfl: 635 nm
Works on fixed cells? yes, PFA and methanol
Probe quantity: 35 nmoles
Fluorescence lifetime: 2.9 ns
STED depletion wavelength: 775 nm
Shipping: room temperature
Storage: -20°C

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