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Overview
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SPY620-actin is a bright red & non toxic live and fixed cells F-actin stain based on our SPY™ dyes series. Its optimized structure allows quick labeling of F-actin in live & fixed cells with high specificity and very low background. SPY620-actin stains F-actin of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the red minimizes phototoxicity and sample autofluorescence. SPY620-actin enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR & GFP. SPY620-actin can be imaged using e.g. 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Contains 1 vial of SPY620-actin (lyophilized).
Absorbance maximum λabs: 619 nm
Fluorescence maximum λfl: 636 nm
Works on fixed cells? yes, PFA fixed cells
Probe quantity: 100 stainings
Fluorescence lifetime: 2.9 ns
STED depletion wavelength: 775 nm
Shipping: room temperature
Storage: -20°CPlease contact us at for specific academic pricing.
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- Properties
- Applications
- Reference
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Overview