SPY555-actin

- Overview
  - SPY555-actin is a bright & non toxic live and fixed cells F-actin stain based on our SPY dyes series. Its optimized structure allows quick labeling of F-actin in live & fixed cells with high specificity and very low background. SPY555-actin stains F-actin of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission are similar to tetramethylrhodamine (TMR). SPY555-actin enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR & GFP. SPY555-actin can be imaged with standard TMR or Cy3 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. Contains 1 vial of SPY555-actin (lyophilized).
    
    Absorbance maximum λabs: 555 nm
    Fluorescence maximum λfl: 580 nm
    Works on fixed cells? yes, PFA fixed cells
    Probe quantity: 100 stainings
    Fluorescence lifetime: 2.4 ns
    STED depletion wavelength: 660 or 775 nm
    Shipping: room temperature
    Storage: -20°C
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Live Cell Imaging Reagents
    
    * For Research Use Only
- Applications
  - Application
    
    Spirochrome Probes for Bioimaging
- Reference
  - Taninaka, Atsushi et al. Direct analysis of the actin-filament formation effect in photodynamic therapy. RSC Advances. 2022
    Schnauß, Jörg et al. Cells in Slow Motion: Apparent Undercooling Increases Glassy Behavior at Physiological Temperatures. Advanced Materials. 2021
    Junqueira Alves, Chrystian et al. Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion. Nature Communications. 2021
    Perez, Jose E. et al. Transient cell stiffening triggered by magnetic nanoparticle exposure. Journal of Nanobiotechnology. 2021
    Wang, Baile et al. The adaptor protein APPL2 controls glucose-stimulated insulin secretion via F-actin remodeling in pancreatic β-cells. Proceedings of the National Academy of Sciences of the United States of America. 2020

Datasheet

MSDS

Comparison of SPY-labeled HeLa cells imaged with confocal vs. STED microscopy. Imaged with 93X objective and provided courtesy of Spirochrome.

SPY555-actin

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