SiR700-BG Substrate

SiR700-BG is the benzylguanine derivative of our SiR700 fluorophore. It emits light in the far-red, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR700-BG can be imaged with a standard Cy5 filterset but using a 700 nm LP emission filter yields better results. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It is identical to SiR700-BG, used in its original publication in JACS.Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion.

*SNAP-tag™ and SNAP-Cell® are registered trademarks of New England Biolabs, Inc.

Absorbance maximum λabs: 696 nm
Fluorescence maximum λfl: 718 nm
Works on fixed cells? yes, PFA and methanol
Probe quantity: 35 nmoles
Fluorescence lifetime: 2.4 ns
STED depletion wavelength: 780-820 nm
Shipping: room temperature
Storage: -20°C

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