Signal-Seeker™ Tricostatin A (TSA) treated and untreated cell lysates

Product Uses
Positive control for anti-acetyl lysine antibodies (Cat. # AAC01)

Material
A431 cells were grown in a CO2 incubator at 37°C to approximately 70% confluency in DMEM media supplemented with 10% fetal bovine serum. Cells were subsequently treated with 0.66 µM TSA in DMSO (TSA treated cells) or with DMSO only (untreated cells) and returned to the incubator for 5h. Cell lysates were subsequently harvested. TSA treated and untreated lysates are supplied as lyophilized powder.

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