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Overview
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Product Uses Include:
Rho signaling pathway studies
Rho activation assays with primary cells
Rho activation assays with limited material
High throughput screens for Rho activation
Introduction
The G-LISA Rho activation assays are ELISA based Rho activation assays with which you can measure Rho activity in cells in less than 3 h. BK124 is very sensitive and has excellent accuracy between duplicate samples. For a more detailed introduction on G-LISA assays and a listing of other available G-LISA kits, see our main G-LISA page. The BK124 Rho activation assay kit measures the level of GTP-loaded RhoA only in cells. The level of activation is measured with absorbance set at 490nm. For a kit to measure RhoA activation with luminescence detection, see Cat. # BK121.Please contact us at for specific academic pricing.
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Properties
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CategoriesActivation Assays
* For Research Use Only
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Reference
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López-Posadas, Rocío et al. Rho-A prenylation and signaling link epithelial homeostasis to intestinal inflammation. Journal of Clinical Investigation. 2016
Yu, Yonghao et al. Hydrogen-rich medium ameliorates lipopolysaccharide-induced barrier dysfunction via rhoa-mdia1 signaling in caco-2 cells. Shock. 2016
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Manukyan, Arkadi et al. A complex of p190RhoGAP-A and anillin modulates RhoA-GTP and the cytokinetic furrow in human cells. Journal of Cell Science. 2015
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Herr, Michael J. et al. Tetraspanin CD9 regulates cell contraction and actin arrangement via RhoA in human vascular smooth muscle cells. PLoS ONE. 2014
Zhu, Ying Ting et al. Knockdown of both p120 catenin and kaiso promotes expansion of human corneal endothelial monolayers via rhoa-rock-noncanonical BMP-NFκB pathway. Investigative Ophthalmology and Visual Science. 2014
Dubash, Adi D. et al. The GEF Bcr activates RhoA/MAL signaling to promote keratinocyte differentiation via desmoglein-1. Journal of Cell Biology. 2013
DiScipio, Richard G. et al. Complement C3a signaling mediates production of angiogenic factors in mesenchymal stem cells. Journal of Biomedical Science and Engineering. 2013
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Yang, Jian et al. Inhibition of farnesyl pyrophosphate synthase attenuates angiotensin II-induced cardiac hypertrophy and fibrosis in vivo. International Journal of Biochemistry and Cell Biology. 2013
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Kalia, Manjula et al. Japanese Encephalitis Virus Infects Neuronal Cells through a Clathrin-Independent Endocytic Mechanism. Journal of Virology. 2013
Kanazawa, Yasushi et al. The Rho-kinase inhibitor fasudil restores normal motor nerve conduction velocity in diabetic rats by assuring the proper localization of adhesion-related molecules in myelinating Schwann cells. Experimental neurology. 2013
Zhou, Zhigang et al. HSV-mediated gene transfer of C3 transferase inhibits Rho to promote axonal regeneration. Experimental Neurology. 2012
Ramsay, Alan G. et al. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenali******: Establishing a reversible immune evasion mechanism in human cancer. Blood. 2012
Chen, Si Meng et al. Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR. Biochemical Pharmacology. 2012
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Zhu, Ying Ting et al. Nuclear p120 catenin unlocks mitotic block of contactinhibited human corneal endothelial monolayers without disrupting adherent junctions. Journal of Cell Science. 2012
Yang, Seungwon et al. The RhoA-ROCK-PTEN pathway as a molecular switch for anchorage dependent cell behavior. Biomaterials. 2012
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Dhaliwal, Anandika et al. Cellular Cytoskeleton Dynamics Modulates Non-Viral Gene Delivery through RhoGTPases. 2012
Greco, Carolina M. et al. Chemotactic effect of prorenin on human aortic smooth muscle cells: a novel function of the (pro)renin receptor. Cardiovascular Research. 2012
Chen, Guang et al. Inhibition of chemokine (CXC motif) ligand 12/chemokine (CXC motif) receptor 4 axis (CXCL12/CXCR4)-mediated cell migration by targeting mammalian target of rapamycin (mTOR) pathway in human gastric carcinoma cells (Journal of Biological Chemistry (2012) 2. Journal of Biological Chemistry. 2012
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Garrido‐Gómez, Tamara et al. Annexin A2 is critical for embryo adhesiveness to the human endometrium by RhoA activation through F‐actin regulation. The FASEB Journal. 2012
Ganguly, Riya et al. Adiponectin Increases LPL Activity via RhoA/ROCK-Mediated Actin Remodelling in Adult Rat Cardiomyocytes. Endocrinology. 2011
Mammoto, Tadanori et al. Mechanochemical Control of Mesenchymal Condensation and Embryonic Tooth Organ Formation. Developmental Cell. 2011
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Yang, Enyue et al. Fluoride induces vascular contraction through activation of RhoA/Rho kinase pathway in isolated rat aortas. Environmental toxicology and pharmacology. 2010
Nini, Lylia et al. Accurate and reproducible measurements of RhoA activation in small samples of primary cells. Analytical biochemistry. 2010
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Hammar, Eva et al. Role of the Rho-ROCK (Rho-Associated Kinase) Signaling Pathway in the Regulation of Pancreatic β-Cell Function. Endocrinology. 2009
Seifert, Jennifer L. et al. Differential activation of Rac1 and RhoA in neuroblastoma cell fractions. Neuroscience letters. 2009
Mercer, Jason et al. Vaccinia virus uses macropinocytosis and apoptotic mimicry to enter host cells. Science (New York, N.Y.). 2008
Kinoshita, Nagatoki et al. Apical Accumulation of Rho in the Neural Plate Is Important for Neural Plate Cell Shape Change and Neural Tube Formation. Molecular Biology of the Cell. 2008
Korobova, Farida et al. Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells. Molecular biology of the cell. 2008
Ramirez, Servio H. et al. Activation of Peroxisome Proliferator-Activated Receptor γ (PPARγ) Suppresses Rho GTPases in Human Brain Microvascular Endothelial Cells and Inhibits Adhesion and Transendothelial Migration of HIV-1 Infected Monocytes. The Journal of Immunology. 2008
Moore, Simon W. et al. Rho inhibition recruits DCC to the neuronal plasma membrane and enhances axon chemoattraction to netrin 1. Development. 2008
Sequeira, Linda et al. Rho GTPases in PC-3 prostate cancer cell morphology, invasion and tumor cell diapedesis. Clinical & experimental metastasis. 2008
Schreibelt, Gerty et al. Reactive oxygen species alter brain endothelial tight junction dynamics via RhoA, PI3 kinase, and PKB signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2007
Rupp, Paul A. et al. A role for RhoA in the two-phase migratory pattern of post-otic neural crest cells. Developmental Biology. 2007
Keely, Patricia J. et al. Investigating integrin regulation and signaling events in three-dimensional systems. Methods in enzymology. 2007
Tanaka, Shigeru et al. Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth. The Journal of biological chemistry. 2007
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Overview