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Overview
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Product Uses Include:
Ras signaling pathway studies
Ras activation assays with primary cells
Studies of Ras activators and inactivators
Ras activation assays with limited material
High throughput screens for Ras activation
Introduction
With the new Ras G-LISA™ kit you can now measure Ras activation from cell and tissue samples in less than 3 h. G-LISA™ requires only 1-5% of the material needed for a conventional pull-down assay. You will also be able to handle large sample numbers and generate quantitative results. For a more detailed introduction on G-LISA™ assays and a listing of other available G-LISA™ kits, see our main G-LISA™ page.
The Ras G-LISA™ kit contains a Ras GTP-binding protein linked to the wells of a 96 well plate. Active, GTP-bound Ras in cell/tissue lysates will bind to the wells while inactive GDP-bound Ras is removed during washing steps. The bound active Ras is detected with a Ras specific antibody. The degree of Ras activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates. Inactivation of Ras is generally achieved in tissue culture by a serum starvation step (see kit datasheet for more information).Please contact us at for specific academic pricing.
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