MGIEasy stLFR Library Prep Kit

MGIEasy stLFR Library Prep Kit* is the world's first partition-less long fragment DNA co-barcoding library prep kit [1]. Based on the principle of DNA co-barcoding, which adds the same barcode sequence to sub-fragments of the original long DNA molecule, stLFR achieves long range genetic information using high throughput and highly precise short read sequencing. Coupled with the MGI DNBSEQTM sequencing platform, stLFR enables high quality small variants calling, phasing of over 99% of the human genome, better genome coverage, detection of large structural variants, and other long read applications.

[1] - Wang et al, 2019. Genome Res.29: 798-808.

Features
• Long range information
Long fragment read lengths of 50-70kb on average, up to 300kb.
• Ultra-low DNA input
Only 1.5ng required, 98% less than single molecule sequencing methods.
• Haplotype phasing
Derive heterozygous variants linkage information for genetic disease and reproductive health applications.
• Single-tube reaction
No need for nanoliter liquid handling or additional purchase of microfluidic systems.
• Automatable library prep
Automatable library prep on the MGISP-960, minimal hands-on time and effort.
• Structural variants detection
Detect copy number variants, deletions, inversions, and balanced translocations.

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