MGIEasy FS PCR-Free DNA Library Prep Set

MGIEasy FS PCR-Free DNA Library Prep Set is specifically designed for constructing WGS libraries without PCR amplification for the MGI high-throughput sequencing platform. For the high-quality and low-bias fragmentation enzyme, the set could fragment 50-1000ng gDNA. The distribution of fragmented DNA is similar to that fragmented by mechanical techniques. The set combined with the automatic sample preparation system MGISP, could provide an automatic library preparation workflow. FS PCR-free combined with MGI DNBSEQTM sequencer, is truly PCR-free sequencing with no amplification errors accumulation.

Features
• Good compatibility for different species fragmentation
Using a high-quality, low bias enzyme for different species and input amounts shearing with the same incubation condition can get the consistent and concentrated fragment range.
• Stable library yield
The modified reaction system increases the library quality and results in a stable library yield.
• Simple and automated workflow
Library construction can be completed within 3.9 hours. Compatible with automatic sample preparation systems to provide an automated solution.
• No amplification error accumulation
WGS PCR-free library preparation in combination with sequencing by the MGI DNBSEQTM platform has no amplification error accumulation, resulting in better genome fidelity.
• Good coverage uniformity
The genome coverage of FS PCR-free achieved comparable performance to Covaris PCR-free. The FS PCR-free technique covers the GC-rich, promoter and repetitive regions. Significantly better than traditional WGS methods.
• Excellent variant performance
The variant detection performance of FS PCR-free is similar to Covaris PCR-free, and better than traditional PCR, especially InDels.

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