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Overview
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LV-EF1 α -SpC as9 is a pre-made lentivirus which over-expresses SpCas9 under the EF1 α promoter and can be used along with guided RNA for genome-editing. The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. Cas9 (CRISPR associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes' CRISPR immunity system. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. Ready to use format.
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