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Overview
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LV-CMV-tdTOMATO-Puro is pre-made lentivirus which expresses tdTOMATO under CMV promoter with co-expression of puromycin selection under PGK promoter. tdTOMATO, tandem dimeric (pseudo-monomeric) derivative of DsRed, is an exceptionally bright red fluorescent protein with around 6 times brighter than EGFP. Because the two subunits of tdTomato are linked covalently, it behaves as a monomer, and has been used successfully for N- and C- terminal fusions. It shows very low aggregation, excellent photostability and its half-time for maturation is just one hour at 37°C. The red emission and brightness of tdTomato’s make it ideal for both in vitro and in vivo imaging studies.
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