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Overview
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Product Uses Include:
Cell invasion assays
FACS analysis of laminin binding cells
Material
Laminin-1 is purified from EHS tumor tissue and is free of the laminin binding protein entactin which is a common contaminant in some laminin preparations (150 kDa). Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% polyacrylamide gel. The laminin is >90% pure.
The protein is modified to contain covalently linked rhodamines at random surface lysines. An activated ester of rhodamine [(5-(and 6)-carboxytetramethylrhodamine succinimidyl ester] is used to label the protein. Labeling stoichiometry is determined by spectroscopic measurement of protein and dye concentrations. Final labeling stoichiometry is 2-5 dyes per protein molecule. The material is guaranteed to contain <15% of free dye and >85% of dye conjugated to laminin. Rhodamine laminin can be detected using a filter set of 535 nm excitation and 585 nm emission.
Laminin runs as individual subunits on SDS-PAGE with an apparent molecular weight of 400 and 225 kDa. LMN01 is supplied as a pale pink lyophilized powder. Each vial of LMN01 contains 20 μg protein.Please contact us at for specific academic pricing.
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- Properties
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Overview