HotBegan™ Hot Start Red-Taq MasterMix

HotBegan™ Hot Start Red-Taq Master Mix (2x) is an optimized ready-to-use solution containing HotBegan™ Hot Start Taq DNA Polymerase, dNTPs, MgCl2 and Stabilizers. It is inactive at room temperature and only requires addition of template, primers, and water. HotBegan™ Hot Start Taq DNA Polymerase is a Taq DNA Polymerase bound to a proprietary antibody that blocks Polymerase activity until denaturation step occurs. The heat labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and nonspecific products. MasterMix also contains an Agarose Loading Buffer including a Red Dye for visual tracking of DNA migration and a dense compound to facilitate the drop-down of the samples into the well Agarose Gels. Like the Taq polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of enzyme activities are detectable.

Includes for 500 rxn:
– 10 x 1. 25 mL HotBegan™ HS Red-Taq MasterMix (2x)*
– 1. 5 mL MgCl2 Solution (50mM)**

Please contact us at for specific academic pricing.